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Novel AAV-ITR Sequencing and AAV Plasmid Preparation Protocols to Advance Research and Therapies Using AAV Vectors


About the tech note

About the tech note

Integrity of the inverted terminal repeat (ITR) regions of adeno-associated virus (AAV) transfer plasmid is crucial for rAAV production. Truncated ITRs can reduce the yield of full viral capsid production and increase generation of undesirable non-rAAV encapsidated DNA. GENEWIZ’s proprietary AAV plasmid preparation process significantly improves the chance of maintaining ITR integrity, and can also isolate clones with full-length ITRs from a sample mixture containing intact and truncated ITRs.

What you'll learn


  • Importance of ITR integrity in rAAV production
  • How GENEWIZ’s proprietary AAV-ITR sequencing method successfully sequences through ITR regions
  • How to best maintain ITR integrity with GENEWIZ’s proprietary AAV plasmid preparation workflow

For technical support questions, please contact our Technical Support team

+1 (877) 436-3949 ext. 2 | DNAseq@genewiz.com