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Tech_Note

Reading Through the Inverted Terminal Repeats (ITRs) of Adeno-Associated Virus (AAV): GENEWIZ's Proprietary Sanger Sequencing Method

The inverted terminal repeat (ITR) regions of adeno-associated virus genomes are important for rAAV production because of their key role in virus replication and encapsidation. These ITRs form highly-stable secondary structures that pose major obstacles for sequence verification using existing technologies. To alleviate this issue, GENEWIZ has developed a new proprietary Sanger sequencing method that allows qualitative assessment of the integrity of these ITR regions.

 

 

What you’ll learn:

  • Importance of ITR regions in rAAV production
  • Challenges of sequencing the AAV-ITR regions
  • How GENEWIZ's direct Sanger sequencing method can sequence the AAV-ITRs

Download the Tech Note

For questions, please contact our Technical Support team

+1 (877) 436-3949 ext. 2 |  DNAseq@genewiz.com