Reading Through the Inverted Terminal Repeats (ITRs) of Adeno-Associated Virus (AAV): GENEWIZ's Proprietary Sanger Sequencing Method

The inverted terminal repeat (ITR) regions of adeno-associated virus genomes are important for rAAV production because of their key role in virus replication and encapsidation. These ITRs form highly-stable secondary structures that pose major obstacles for sequence verification using existing technologies. To alleviate this issue, GENEWIZ has developed a new proprietary Sanger sequencing method that allows qualitative assessment of the integrity of these ITR regions.

What you’ll learn:

• Importance of ITR regions in rAAV production
• Challenges of sequencing the AAV-ITR regions
• How GENEWIZ's direct Sanger sequencing method can sequence the AAV-ITRs