De Novo Synthesis of a 150 kb Construct

De Novo Synthesis of a 150 kb Construct

Building and manipulating large DNA molecules pose several complications for de novo synthesis and sequence verification. In this application note, we discuss how our established difficult template protocols enable accurate construction of even the most challenging regions.

What You'll Learn

• Challenges of large construct assembly
• How our proprietary protocols successfully synthesize large constructs
• Importance of transformation-associated recombination (TAR) cloning in the construct assembly process
• Insights into how we synthesize molecules of any size or complexity, from single genes to chromosome-scale structures, that are tailored to meet your unique needs