High-Throughput Genotyping Case Study

Rapid, Scalable, and Ultra-Sensitive Validation of Allele-Specific Gene Editing Events with genoTYPER-NEXT™

This case study highlights a collaboration between GENEWIZ from Azenta Life Sciences and a large pharmaceutical company to develop a more effective and scalable approach for screening engineered cell lines using an automated, interactive, NGS-based genotyping platform. This powerful solution enables high-resolution identification of genome editing outcomes, including mono-allelic, homozygous, and heterozygous bi-allelic gene editing events, while significantly reducing manual data handling and interpretation time.

What you'll learn

• How NGS-based genoTYPER-NEXT™ streamlines high-throughput screening of engineered cell lines
• The limitations of traditional mutation detection and Sanger-based workflows for bi-allelic gene editing validation
• How automated, interactive data visualization simplifies interpretation of CRISPR, TALEN, and ZFN editing outcomes
• The advantages of ultra-sensitive detection for identifying rare and complex INDEL mutation patterns