---
title: A Guide to RNA-Seq | GENEWIZ from Azenta
description: Learn more about RNA sequencing with our eBook, which explores one of the most popular next generation sequencing methods for analyzing the transcriptome.
---

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- [English](https://web.genewiz.com/ebook/rna-seq-guide)
- [日本語 - 日本](https://web.genewiz.com/ja-jp/ebook/rna-seq-guide)

 English

# A Guide to RNA-Seq

An in-depth guide for achieving success in your RNA-Seq experiments.

eBOOK

## RNA Sequencing Guide

RNA sequencing (RNA-Seq) is a powerful tool to help researchers reveal novel biological insights into the transcriptome. This eBook allows you to dive deeper into this popular NGS technique by covering the basics of RNA-Seq, the complete experimental process, data analysis, and tips to help you achieve the best results for your own RNA-Seq experiment.

![RNA-Seq eBook - Page 1](https://3478602.fs1.hubspotusercontent-na1.net/hub/3478602/hubfs/2025-12%20GEN%20NGS%20-%20RNA-Seq%20eBook%20Refresh/RNA-Seq%20eBook%201.png?width=1200&length=1200&name=RNA-Seq%20eBook%201.png)

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![RNA-Seq eBook - Page 4](https://3478602.fs1.hubspotusercontent-na1.net/hub/3478602/hubfs/2025-12%20GEN%20NGS%20-%20RNA-Seq%20eBook%20Refresh/RNA-Seq%20eBook%20-%20Page%204.png?width=1200&length=1200&name=RNA-Seq%20eBook%20-%20Page%204.png)

![RNA-Seq eBook - Page 5](https://3478602.fs1.hubspotusercontent-na1.net/hub/3478602/hubfs/2025-12%20GEN%20NGS%20-%20RNA-Seq%20eBook%20Refresh/RNA-Seq%20eBook%20-%20Page%205.png?width=1200&length=1200&name=RNA-Seq%20eBook%20-%20Page%205.png)

### What You’ll Learn

- **The fundamentals of RNA-Seq,** including transcriptome anatomy, different methodologies, and each step of a typical project workflow
- **Bioinformatics analysis** and essential tools to help you extract biologically relevant information and maximize your data output
- **Key considerations for experimental design,** including library construction, sequencing technology, and more
- **Leveraging ultra-low input RNA-Seq** to uncover rare expression profiles in the tumor microenvironment

[Read Chapter One](https://blog.genewiz.com/rna-seq-workflows-methods-guide?hsLang=en)

## Download eBook