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A Guide to Single-Cell Sequencing

The ultimate resource for optimizing your single-cell project.

eBOOK

Single-Cell Sequencing Guide

Single-cell sequencing is a valuable tool for analyzing complex cellular populations, enabling high-resolution views of the transcriptome, epigenome, and immune repertoire that are not possible with conventional bulk sequencing. This eBook explores the fundamentals of single-cell sequencing, focusing on how the technology works, best-practice approaches to enhance results, and real-world applications to help you get the most out of your single-cell research.

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What You'll Learn

  • The basics of single-cell sequencing, including the most common types of single-cell sequencing techniques such as single-cell RNA-Seq, single-cell ATAC-Seq (assay for transposase-accessible chromatin by sequencing), single-cell immune profiling, and single-cell multiomics (i.e., RNA-Seq + ATAC-Seq), as well as key considerations for study design
  • Leveraging dead cell removal to overcome low cell viability and generate high-quality single-cell sequencing results
  • Alternate cell fixation methods for preparing single-cell samples without sacrificing integrity or data quality.
  • Using single-cell RNA sequencing to identify rare cells, characterize their transcriptomes, and discover potential biomarkers
  • High-resolution spatial profiling and biomarker discovery using spatial transcriptomics

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