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For a limited time, get 25% off your ssDNA synthesis order 


GENEWIZ Single-Stranded DNA (ssDNA) synthesis service provides fast and affordable, sequence-verified long ssDNA fragments between 151 and 5,000 nt. ssDNA fragments are derived from clonally purified double-stranded DNA, resulting in the highest possible quality. This increases the likelihood of homology directed repair (HDR) with reduced cellular toxicity. As the leader in working with complex gene sequences, you can trust GENEWIZ to deliver sequence-verified fragments for CRISPR-mediated gene knock-in, in vitro transcription and many more applications.

Pricing and Turnaround Time

DNA Length Quantity (Lyophilized) Turnaround Time Price
151-500 nt 3μg or 10μg* or 20μg* 15-20 business days

€409 €280
£319 £240

501-3000 nt 20-25 business days

€0.83/nt €0.56/nt

3001-5000 nt 25-30 business days

€0.83/nt €0.56/nt
£0.65/nt £0.49/nt

*Additional charges apply for 10μg and 20μg quantities.

CRISPR-Mediated Gene Knock-In Workflow



ssDNA Synthesis Applications

check.png  CRISPR-Mediated Gene Knock-In

check-mark-small.png  In Vitro Transcription

check-mark-small.png  SNP Detection

check-mark-small.png  DNA Aptamer Production check-mark-small.png  Subtractive Hybridization check-mark-small.png  Nuclease S1 Mapping



For technical support questions, please contact our Project Management team

+49 (0)341 520 122–0   |    gs.europe@genewiz.com

Terms and Conditions: Promotional discount applies to Single-Stranded DNA synthesis services only. Order must be submitted via online request form or Excel upload. Discounts cannot be combined with any other offers or promotions, including GENEWIZ Referral Rewards. Promotion not valid for U.S. production or TurboGENE. Promotion code required to receive discount; retroactive application is prohibited. Promotions subject to change anytime without notice. Other restrictions may apply. Offer expires June 30, 2019.