A Guide to RNA-Seq

What You’ll Learn

• The fundamentals of RNA-Seq, including transcriptome anatomy, different methodologies, and each step of a typical project workflow
• Bioinformatics analysis and essential tools to help you extract biologically relevant information and maximize your data output
• Key considerations for experimental design, including library construction, sequencing technology, and more
• Leveraging ultra-low input RNA-Seq to uncover rare expression profiles in the tumor microenvironment