Single-Cell Sequencing Sample Preparation Guide

Single-Cell Sequencing Sample Preparation

Depending on the goals of your single-cell sequencing experiment and the library preparation chemistries being used, cells and tissues may be fixed or cryopreserved prior to shipment. Proper sample preparation is critical to preserving cell viability, sample integrity, and data quality for downstream single-cell RNA-seq, immune profiling, and multiomic workflows.

This guide provides practical, step-by-step single-cell sample preparation workflows to support successful processing at GENEWIZ. It outlines recommended approaches for preserving cells, nuclei, and tissues prior to shipment, with guidance on minimum cell numbers, compatibility with common 10x Genomics chemistries, and best practices to reduce sample loss during transport.

This guide covers:

• Cryopreservation of dissociated cells to maintain viability for single-cell sequencing and dead-cell removal workflows
• Fixation of dissociated cells and nuclei for compatible probe-based and fixed RNA profiling assays
• ixation of intact tissue to support downstream dissociation and single-cell analysis while maintaining sample stability