--- title: Single-Cell Sequencing Sample Preparation Guide | GENEWIZ description: Discover essential single-cell sequencing sample preparation techniques to help maintain cell viability when shipping your samples to GENEWIZ. --- [Skip to content](https://web.genewiz.com/guide/single-cell-sample-prep#main-content) [![GENEWIZ](https://web.genewiz.com/hubfs/GENEWIZ%20Brand%20Assets/GENEWIZ%20Logos/2025-04%20Current%20GENEWIZ%20Logos/GENEWIZ_Full-Color_Logo_PublicWeb_200x63.svg "GENEWIZ")](https://www.genewiz.com/) English # Single-Cell Sequencing Sample Preparation Guide GUIDE ## Single-Cell Sequencing Sample Preparation Depending on the goals of your **single-cell sequencing experiment** and the **library preparation chemistries** being used, cells and tissues may be **fixed or cryopreserved** prior to shipment. Proper sample preparation is critical to preserving **cell viability, sample integrity, and data quality** for downstream **single-cell RNA-seq, immune profiling, and multiomic workflows**. This guide provides practical, step-by-step **single-cell sample preparation workflows** to support successful processing at GENEWIZ. It outlines recommended approaches for preserving cells, nuclei, and tissues prior to shipment, with guidance on minimum cell numbers, compatibility with common **10x Genomics chemistries**, and best practices to reduce sample loss during transport. ### This guide covers: - **Cryopreservation of dissociated cells** to maintain viability for single-cell sequencing and dead-cell removal workflows - **Fixation of dissociated cells and nuclei** for compatible probe-based and fixed RNA profiling assays - **ixation of intact tissue** to support downstream dissociation and single-cell analysis while maintaining sample stability ![Single-Cell Sample Prep Guide - LP Thumbnail](https://web.genewiz.com/hs-fs/hubfs/2025-09%20GEN%20NGS%20-%20Single-Cell%20eBook/Single-Cell%20Sample%20Prep%20Guide%20-%20LP%20Thumbnail-2.png?width=1600&height=1810&name=Single-Cell%20Sample%20Prep%20Guide%20-%20LP%20Thumbnail-2.png "Single-Cell Sample Prep Guide - LP Thumbnail") ## Download Guide ## Related Services [Single-cell Sequencing](https://www.genewiz.com/public/services/next-generation-sequencing/illumina-sequencing) High-resolution transcriptomic profiling from individual cells to reveal cellular heterogeneity [Single-cell Library Sequencing Only](https://www.genewiz.com/public/services/next-generation-sequencing/illumina-sequencing) High-quality sequencing of pre-prepared single-cell libraries with fast, reliable data delivery. [RNA Sequencing](https://www.genewiz.com/public/services/next-generation-sequencing/whole-genome-sequencing) Comprehensive transcriptome profiling to quantify gene expression and transcript diversity.