Peripheral blood mononuclear cells (PBMCs) are a valuable sample type for basic research and clinical studies. The quantity and quality of these isolated cells are critical to the performance of functional assays, as poor sample handling may result in inaccurate data. In this tech note, we discuss the challenges associated with PBMC isolation and offer a robust workflow for PBMC isolation and cryopreservation, ensuring high-quality results that exceed industry standards for cell viability and yield.

What you'll learn

• The importance of quantity and quality of isolated PBMC cells for the downstream analysis
• The challenges in preanalytical steps of PBMC isolation that affect sample quality
• How Brooks Life Sciences and GENEWIZ optimized workflows for the rapid isolation and freezing of PBMCs with reproducible, best-in-class results