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Simply submit your bacterial colonies as agar plates or glycerol stocks and select to save the submitted sample(s) for a future plasmid preparation order within the Sanger sequencing order form. Download the how to order guide for details. 

 

Direct Colony Sequencing + Plasmid DNA Preparation Workflow

GENEWIZ’s streamlined direct colony sequencing and plasmid DNA preparation workflow utilizes rolling circle amplification (RCA) prior to Sanger sequencing to expedite screening and prep-up your choice of positive clones to any scale.

 

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Step 1

Conduct in-house experiments and submit agar plates to GENEWIZ.

 

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Step 2

GENEWIZ performs direct colony sequencing from bacterial clones.

 

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Step 3

Sanger data is available within 24 hours*; review data and select for positive clones.

 

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Step 4

GENEWIZ performs plasmid DNA prep on selected clones starting at 2 business days*.

 


FEATURES & BENEFITS

  • Work with one provider throughout your screening project

  • Save time and resources for your lab

  • Place orders for multiple services seamlessly with our online platform

  • From bacterial colonies to plasmid prep starting at 3 business days*

  • Ph.D.-level customer support through your entire project

 

Service Details

Direct Colony Sequencing

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Utilizes rolling circle amplification (RCA) to enable Sanger sequencing of bacterial clones without the need for plasmid preparation.

Turnaround Time:
24 hours*

Starting Materials:
Bacterial colonies, glycerol
stock

High Quality:
>600 bases of Phred20 reads


Learn More

Plasmid DNA Preparation

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Delivers high quality DNA from a variety of starting materials. Project customization available with various add-on options.

Turnaround Time:
Starting at 2-3 business days

Starting Materials:
Bacterial colonies, DNA,
glycerol stock

Scales:
Mini to Giga


Learn More

 

Ph.D. experts are available to discuss your project.
Call us at 877-436-3949, ext 2 or email dnaseq@genewiz.com.


*24-hour turnaround time is guaranteed for up to 96 reactions. 2 business days valid for orders without add-on analysis options, processed at local GENEWIZ labs