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Simply submit your bacterial colonies as agar plates or glycerol stocks and select to save the submitted sample(s) for a future plasmid preparation order within the Sanger sequencing order form. Download the how to order guide for details. 

 

Direct Colony Sequencing + Plasmid DNA Preparation Workflow

GENEWIZ’s streamlined direct colony sequencing and plasmid DNA preparation workflow utilizes rolling circle amplification (RCA) prior to Sanger sequencing to expedite screening and prep-up your choice of positive clones to any scale.

 

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Step 1

Conduct in-house experiments and submit agar plates to GENEWIZ.

 

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Step 2

GENEWIZ performs direct colony sequencing from bacterial clones.

 

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Step 3

Sanger data is available within 24 hours*; review data and select for positive clones.

 

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Step 4

GENEWIZ performs plasmid DNA prep on selected clones starting at 2 business days*.

 

For a limited time, get one free upgrade to colony sequencing + 25% OFF plasmid prep of your selected clones! Complete the form below to claim your promo codes.

 


FEATURES & BENEFITS

  • Work with one provider throughout your screening project

  • Save time and resources for your lab

  • Place orders for multiple services seamlessly with our online platform

  • From bacterial colonies to plasmid prep starting at 3 business days*

  • Ph.D.-level customer support through your entire project

CLAIM SERVICE DISCOUNTS


 

Service Details

Direct Colony Sequencing

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Utilizes rolling circle amplification (RCA) to enable Sanger sequencing of bacterial clones without the need for plasmid preparation.

Turnaround Time:
24 hours*

Starting Materials:
Bacterial colonies, glycerol
stock

High Quality:
>600 bases of Phred20 reads


Learn More

Plasmid DNA Preparation

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Delivers high quality DNA from a variety of starting materials. Project customization available with various add-on options.

Turnaround Time:
Starting at 2-3 business days

Starting Materials:
Bacterial colonies, DNA,
glycerol stock

Scales:
Mini to Giga


Learn More

 

Ph.D. experts are available to discuss your project.
Call us at 877-436-3949, ext 2 or email dnaseq@genewiz.com.


Terms & Conditions: Offers expire June 30, 2021 and are single use only. One free upgrade to direct colony sequencing is valued up to $200; applies to samples processed at U.S. laboratories only. 25% off plasmid preparation is valid on one order of standard and targeted yield preps; excludes AAV plasmid preparation. Maximum discount is $2,000. Promotion codes must be entered using the online ordering form to be valid. Terms and conditions are subject to change at any time without notice. Other restrictions may apply. Cannot be combined with other offers including discounted pricing contracts, current promotions, and GENEWIZ Referral Rewards.


*24-hour turnaround time is guaranteed for up to 96 reactions. 2 business days valid for orders without add-on analysis options, processed at local GENEWIZ labs