DIRECT COLONY SEQUENCING to Prep Workflow

Step 1

Conduct in-house experiments and submit agar plates to GENEWIZ. 

Step 2

GENEWIZ performs direct colony sequencing from bacterial clones.

Step 3

Sanger data is available within 24 hours*; review data and select for positive clones.

Step 4

GENEWIZ performs plasmid DNA prep on selected clones within 2-3 business days.

Features and Benefits

Individual Service Details

Direct Colony Sequencing

Utilizes rolling circle amplification to enable Sanger sequencing of bacterial clones without the need for plasmid preparation.

Turnaround Time:
24 hours*

Starting Materials:
Bacterial colonies, glycerol stock

High Quality:
>600 bases of Phred20 reads

Plasmid DNA Preparation

Delivers high quality DNA from a variety of starting materials. Project customization available with various add-on options.

Turnaround Time:
Starting at 2-3 business days

Starting Materials:
Bacterial colonies, DNA, glycerol stocks

Scales:
Mini to Giga