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DIRECT COLONY SEQUENCING to Prep Workflow

 

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Step 1

Conduct in-house experiments and submit agar plates to GENEWIZ. 

 

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Step 2

GENEWIZ performs direct colony sequencing from bacterial clones.

 

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Step 3

Sanger data is available within 24-36 hours; review data and select for positive clones.

 

plasmid-prep

Step 4

GENEWIZ performs plasmid DNA prep on selected clones within 2-3 business days.

 



 

ready, set, order!


 

Not sure how to order? Follow these easy steps!

 

 


 

Features and Benefits

    Work with one provider throughout your screening process
    Save time and resources for your lab
Place orders for multiple services seamlessly with our online platform
Turnaround time starts at 3 business days

 

Individual Service Details

Direct Colony Sequencing

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Utilizes rolling circle amplification to enable Sanger sequencing of bacterial clones without the need for plasmid preparation.

 Turnaround Time:

24 - 36 hours

Starting Materials:

Bacterial colonies, glycerol stock, phage supernatant/plaque

High Quality:

>600 bases of Phred20 reads


Learn More

Plasmid DNA Preparation

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Delivers high quality DNA from a variety of starting materials. Project customization available with various add-on options.

 Turnaround Time:

Starting at 2-3 business days

Starting Materials:

Bacterial colonies, DNA,
glycerol stocks

Scales:

Mini to Giga


Learn More


GENEWIZ also offers linear dsDNA fragments and oligonucleotides for your upstream screening applications

 

 


 

The GENEWIZ Difference

 

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Ph.D. experts are available to discuss your project.

Call us at 877-436-3949, ext 2 or email dnaseq@genewiz.com.