DIRECT COLONY SEQUENCING to Prep Workflow

Step 1

Conduct in-house experiments and submit agar plates to GENEWIZ. 

Step 2

GENEWIZ performs direct colony sequencing from bacterial clones.

Step 3

Sanger data is available within 24 hours*; review data and select for positive clones.

Step 4

GENEWIZ performs plasmid DNA prep on selected clones within 2-3 business days.

Features and Benefits

Individual Service Details

Direct Colony Sequencing

Utilizes rolling circle amplification (RCA) to enable Sanger sequencing of bacterial clones without the need for plasmid preparation.

Turnaround Time:
24 hours*

Starting Materials:
Bacterial colonies, glycerol
stock

High Quality:
>600 bases of Phred20 reads

Plasmid DNA Preparation

Delivers high quality DNA from a variety of starting materials. Project customization available with various add-on options.

Turnaround Time:
Starting at 2-3 business days

Starting Materials:
Bacterial colonies, DNA, glycerol stock

Scales:
Mini to Giga