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Tech Note

Add More Life to Your Data: Optimizing Single-Cell RNA-Seq with Dead Cell Removal

What you'll learn

Obtaining samples with high cell viability can be difficult for many experiments but is necessary for success on the 10x Genomics® Chromium™ platform. This tech note describes how GENEWIZ scientists used optimized single-cell workflows, including dead cell removal, to overcome low viability and generate high-quality sequencing data.

 

Overview of GENEWIZ’s workflow using the 10x Genomics Chromium system

Challenges of analyzing samples with low cell viability for single-cell sequencing

How dead cell removal can improve sample quality and maximize data richness

Graphical Abstract:

Graphical abstract of GENEWIZ's workflow using the 10x Genomics Chromium

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For technical support questions, please contact our Next Generation Sequencing team

(877) 436-3949 ext. 1 | NGS@genewiz.com