GENEWIZ AAV-ITR Sanger Sequencing Tech Note

Reading Through the Inverted Terminal Repeats (ITRs) of Adeno-Associated Virus (AAV): GENEWIZ AAV-ITR Sanger Sequencing Method

The inverted terminal repeat (ITR) regions of adeno-associated virus genomes are important for rAAV production because of their key role in virus replication and encapsidation. These ITRs form highly-stable secondary structures that pose major obstacles for sequence verification using existing technologies. To alleviate this issue GENEWIZ from Azenta has developed a new proprietary Sanger sequencing method that allows qualitative assessment of the integrity of these ITR regions.

Azenta’s proprietary Sanger sequencing protocol is now GLP-compliant.