An Efficient and Reliable Approach to AAV Packaging Tech Note

An Efficient and Reliable Approach to AAV Packaging

Previous reports have shown that ITRs are necessary to produce effective viral particles, but their structure is problematic for conventional sequencing, synthesis, and cloning workflows. Here, we study the effect of partial and full ITR deletions on AAV packaging and show how the proprietary methods developed by GENEWIZ from Azenta Life Sciences can successfully detect and repair these mutations.

What you'll learn:

• Challenges associated with mutations in AAV plasmid ITR regions
• How partial and full ITR deletions affect cell transduction with AAV particles
• The proprietary methods developed by GENEWIZ to successfully detect, repair, and suppress these mutations