Codon Optimization Tech Note

Strategies for Codon Optimization to Maximize Gene Expression and Protein Yield

Codon optimization is essential for recombinant protein expression and synthetic gene design. Variations in codon preference among host organisms can greatly impact foreign gene expression. By matching codon usage to host preferences, researchers can achieve higher protein yields and more efficient gene synthesis. This technical note highlights two codon optimization case studies, comparing wildtype genes to GENEWIZ’s codon-optimized sequences to demonstrate measurable gains in expression efficiency and cloning accuracy.

Researchers can leverage codon optimization tools to:

• Maximize protein expression in bacterial, mammalian, and yeast hosts through optimized codon usage.
• Accelerate gene synthesis by adjusting GC content and minimizing secondary structures.
• Prevent cloning errors by removing direct, inverted, and dyad repeat sequences.
• Ensure vector compatibility by preserving essential restriction sites during design.