--- title: Full-Length RNA-Seq Tech Note | GENEWIZ from Azenta Life Sciences description: In this tech note, discover the science behind this novel assay and how it’s an optimal approach for quality control for the development of your mRNA-based vaccines or drugs. --- [Skip to content](https://web.genewiz.com/tech-note/full-length-rna-seq#main-content) [![GENEWIZ](https://web.genewiz.com/hubfs/GENEWIZ%20Brand%20Assets/GENEWIZ%20Logos/2025-04%20Current%20GENEWIZ%20Logos/GENEWIZ_Full-Color_Logo_PublicWeb_200x63.svg "GENEWIZ")](https://www.genewiz.com/) English # Full-Length RNA-Seq Tech Note TECH NOTE ## Full-Length RNA-Seq: A Novel Method to Assess Sequence Integrity for RNA Therapeutics The use of **RNA therapeutics** as a gene therapy approach continues to expand due to the ability to **target specific genes** and introduce **defined RNA transcripts**. To develop these therapies, **strict quality control** is required to ensure the integrity of **poly(A) tails**, which are essential for **mRNA stability and translation efficiency**. However, current assays for measuring poly(A) tails present important limitations, including **inaccurate length detection**, limited resolution of mixed populations, and the introduction of technical artifacts. Using **NGS-based PacBio® Small-Molecule, Real-Time (SMRT®) sequencing, GENEWIZ from Azenta Life Sciences** has developed a **high-throughput, full-length RNA sequencing (RNA-Seq) workflow** that enables sequencing of manufactured RNA products while preserving the **full-length poly(A) tail**. In this tech note, explore the science behind this **novel full-length RNA-Seq assay** and learn how it provides an **optimal quality control approach** for the development of **mRNA-based vaccines and RNA therapeutics**.   ### What You'll Learn - Key **challenges associated with sequencing full-length RNA molecules** using traditional analytical approaches - How the **full-length RNA-Seq workflow from GENEWIZ** enables accurate sequencing of **manufactured RNA products** while preserving poly(A) tail integrity - Results from **internal validation studies** confirming **quantification accuracy, reproducibility, and consistency** of the **full-length RNA-Seq assay** ![13002-ATN LP Thumbnail](https://web.genewiz.com/hs-fs/hubfs/TECH%20NOTES%202026/13002-ATN%20LP%20Thumbnail.png?width=884&height=1000&name=13002-ATN%20LP%20Thumbnail.png "13002-ATN LP Thumbnail") ## Download Tech Note ## Related Services [Long-read Isoform Sequencing](https://www.genewiz.com/public/services/next-generation-sequencing/iso-seq) Full-length transcript sequencing to resolve isoforms and complex splicing. [RNA Sequencing](https://www.genewiz.com/public/services/next-generation-sequencing/whole-genome-sequencing) Comprehensive transcriptome profiling to quantify gene expression and transcript diversity. [Whole Genome Sequencing](https://www.genewiz.com/public/services/next-generation-sequencing/whole-genome-sequencing) High-coverage sequencing of complete genomic DNA.