High-Fidelity Production of In Vitro Transcription Plasmids with Long Poly(A) Sequences Tech Note

High-Fidelity Production of In Vitro Transcription Plasmids with Long Poly(A) Sequences

Due to their versatility for protein expression, synthetic mRNA plasmids have become an ideal tool for gene therapies. A key component of an mRNA plasmid is the poly(A) tail, which determines the structural integrity of the molecule. While long poly(A) sequences have generally resulted in increased stability of mRNA products, poly(A) tails with greater than 100 bases can be susceptible to truncations during production, resulting in unstable mRNA products.

To address this challenge, GENEWIZ from Azenta Life Sciences has developed an efficient protocol using a proprietary E. coli strain and optimized culturing methods to ensure long poly(A) sequence stability of at least 140 bases in mRNA plasmids. In this tech note, discover how this method can help you achieve high-fidelity templates for in vitro transcription (IVT).

What you'll learn:

• How synthetic mRNA plasmids are developed for IVT
• Benefits and challenges of synthetic mRNA plasmid preparation
• Comparison of standard preparation protocols and the proprietary protocol from Azenta