--- title: Visium HD Tech Note | GENEWIZ from Azenta description: Explore how Visium HD® bridges the gap between histology and gene expression profiling, offering precise biomarker discovery and cellular mapping within complex tissues. --- [Skip to content](https://web.genewiz.com/tech-note/high-resolution-discovery-visium-hd#main-content) [![GENEWIZ](https://web.genewiz.com/hubfs/GENEWIZ%20Brand%20Assets/GENEWIZ%20Logos/2025-04%20Current%20GENEWIZ%20Logos/GENEWIZ_Full-Color_Logo_PublicWeb_200x63.svg "GENEWIZ")](https://www.genewiz.com/) English # High-Resolution Spatial Profiling and Biomarker Discovery by Visium HD TECH NOTE ## High-Resolution Spatial Profiling and Biomarker Discovery with Visium HD Despite significant advancements brought by traditional **RNA sequencing** methods, important limitations remain when it comes to **spatial gene expression analysis**. Bulk RNA-seq lacks spatial context, while single-cell RNA-seq sacrifices tissue architecture, both creating gaps in biological interpretation. Here, we explore how the **10X Genomics Visium HD® spatial transcriptomics platform** bridges the gap between **histology and gene expression profiling**, enabling high-resolution **spatial profiling**, precise **biomarker discovery**, and detailed **cellular mapping** within complex tissues. This approach supports deeper biological insight across **oncology, immunology, and developmental biology research**. ### What You'll Learn - Learn how 10X Genomics **Visium HD spatial transcriptomics** enables comprehensive **spatial characterization of tissues** while preserving cellular context. - Explore a **qualification study** demonstrating high **specificity, sensitivity, and consistency in cell type identification** across technical replicates. - Understand how **high-resolution gene expression mapping** improves the identification of **spatially specific biomarkers**, providing deeper insight into **tumor microenvironments, immune cell localization, and developmental processes**. ![13003-ATN LP Thumbnail](https://web.genewiz.com/hs-fs/hubfs/TECH%20NOTES%202026/13003-ATN%20LP%20Thumbnail.png?width=884&height=1000&name=13003-ATN%20LP%20Thumbnail.png "13003-ATN LP Thumbnail") ## Download Tech Note ## Related Services [Spatial Transcriptomics](https://www.genewiz.com/public/services/next-generation-sequencing/spatial-transcriptomics) Map gene expression within intact tissue to preserve spatial context. [Single-cell Sequencing](https://www.genewiz.com/public/services/next-generation-sequencing/illumina-sequencing) High-resolution transcriptomic profiling from individual cells to reveal cellular heterogeneity [RNA Sequencing](https://www.genewiz.com/public/services/next-generation-sequencing/whole-genome-sequencing) Comprehensive transcriptome profiling to quantify gene expression and transcript diversity.