Phenotypic Profiling for Drug Discovery with High-Throughput Gene Expression Screening

Drug discovery applications require reliable measurement of phenotypic responses via high-throughput approaches utalizing ultrafast RNA-Seq and 3' end couting. Traditional approaches such as microarrays and RNA sequencing present inherent limitations. In this tech note, we introduce high-throughput gene expression (HT-GEx) screening, an optimized assay developed by GENEWIZ from Azenta Life Sciences that utilizes cell lysate instead of purified RNA to perform rapid, unbiased phenotypic profiling at a reduced cost - an ideal approach for drug discovery, compound treatment phenotyping, CRISPR treatment phenotyping, and cell response screening.

What you'll learn

• Key challenges of phenotypic profiling in drug discovery when using traditional screening and sequencing approaches
• How GENEWIZ’s HT-GEx screening workflow leverages cell lysate samples to improve throughput, reduce cost, and minimize bias
• Results from sensitivity and reproducibility studies comparing purified RNA versus cell lysate samples in high-throughput gene expression screening