Phenotypic Profiling for Drug Discovery with High-Throughput Gene Expression Screening

Drug discovery applications require reliable measurement of phenotypic responses via high-throughput approaches; however, traditional approaches such as microarrays and RNA sequencing present inherent limitations. In this tech note, we introduce high-throughput gene expression (HT-GEx) screening, an optimized assay developed by Azenta Life Sciences that utilizes cell lysate instead of purified RNA to perform rapid, unbiased phenotypic profiling at a reduced cost - an ideal approach for drug discovery, compound treatment phenotyping, CRISPR treatment phenotyping, and cell response screening.

What you'll learn

• Challenges associated with phenotypic profiling for drug discovery utilizing traditional approaches
• Azenta Life Sciences’ optimized HT-GEx screening workflow utilizing cell lysate samples
• Results from sensitivity and reproducibility tests comparing the use of RNA versus cell lysate samples