Phenotypic Profiling for Drug Discovery with High-Throughput Gene Expression Screening
TECH NOTE
Achieving Phenotypic Profiling for Rapid Drug Discovery with High-Throughput Gene Expression Screening
Drug discovery applications require reliable measurement of phenotypic responses via high-throughput approaches; however, traditional approaches such as microarrays and RNA sequencing present inherent limitations. In this tech note, we introduce high-throughput gene expression (HT-GEx) screening, an optimized assay developed by Azenta Life Sciences that utilizes cell lysate instead of purified RNA to perform rapid, unbiased phenotypic profiling at a reduced cost - an ideal approach for drug discovery, compound treatment phenotyping, CRISPR treatment phenotyping, and cell response screening.
What you'll learn
- Challenges associated with phenotypic profiling for drug discovery utilizing traditional approaches
- Azenta Life Sciences’ optimized HT-GEx screening workflow utilizing cell lysate samples
- Results from sensitivity and reproducibility tests comparing the use of RNA versus cell lysate samples
![technote technote](https://web.genewiz.com/hs-fs/hubfs/technote.png?width=501&height=645&name=technote.png)