mRNA Synthesis Tech Note

mRNA Synthesis via Integrated Gene Synthesis and In Vitro Transcription

Discover best practices for generating high-quality mRNA transcripts through an integrated gene-to-mRNA synthesis workflow. In this technical note, GENEWIZ from Azenta Life Sciences demonstrates how integrated gene synthesis and in vitro transcription (IVT) enable rapid, accurate, and reproducible mRNA synthesis for research and therapeutic development. It outlines proven strategies to optimize mRNA design, transcription, and quality validation, ensuring reliable performance in mRNA-based research, protein expression, and gene therapy applications.

What You’ll Learn

• Critical factors influencing successful mRNA synthesis and transcript yield
• How optimized vectors improve in vitro transcription efficiency
• Best practices for mRNA quality control and validation
• Insights into GENEWIZ’s streamlined gene-to-mRNA workflow for reproducible results