Optimizing Single Cell RNA-Sequencing with Dead Cell Removal

Obtaining samples with high cell viability can be difficult for many experiments, but is necessary for success on the 10x Genomics® Chromium™ platform.

This tech note describes how GENEWIZ from Azenta scientists used optimized single-cell workflows, including dead cell removal, to overcome low viability and generate high-quality sequencing data.

Azenta experts will cover best practices, as well as the following topics:

• Overview of GENEWIZ’s workflow using the 10x Genomics Chromium system
• Challenges of analyzing samples with low cell viability for single-cell sequencing
• How dead cell removal can improve sample quality and maximize data richness