--- title: Single-Cell RNA-Seq Dead Cell Removal Tech Note | GENEWIZ from Azenta description: GENEWIZ scientists optimized single-cell workflows, including dead cell removal, to overcome low viability and generate high-quality sequencing data. image: https://web.genewiz.com/hubfs/single%20dcr.png --- [Skip to content](https://web.genewiz.com/tech-note/single-cell-sequencing-dead-cell-removal#main-content) [![GENEWIZ](https://web.genewiz.com/hubfs/GENEWIZ%20Brand%20Assets/GENEWIZ%20Logos/2025-04%20Current%20GENEWIZ%20Logos/GENEWIZ_Full-Color_Logo_PublicWeb_200x63.svg "GENEWIZ")](https://www.genewiz.com/) English # Optimizing Single Cell RNA-Sequencing with Dead Cell Removal TECH NOTE ## Single Cell RNA-Sequencing with Dead Cell Removal for Improved Data Quality and Cell Viability Obtaining samples with **high cell viability** can be challenging for many experimental workflows, yet it is critical for generating reliable results on the **10x Genomics® Chromium™ single-cell sequencing platform**. Low viability samples often lead to reduced cell recovery, increased background noise from ambient RNA, and compromised data quality. This tech note describes how GENEWIZ from Azenta Life Sciences applied **optimized single-cell RNA-sequencing workflows**, including **dead cell removal**, to overcome low viability constraints and consistently generate **high-quality single-cell RNA-Seq data** from challenging samples. What you’ll learn - An overview of **GENEWIZ’s end-to-end single-cell RNA-Seq workflow** using the **10x Genomics Chromium system** - Key **challenges associated with low cell viability** in single-cell sequencing experiments, including ambient RNA contamination and reduced target cell recovery - How **dead cell removal** can **improve sample quality**, increase **cell viability**, and **maximize data richness** without compromising gene detection sensitivity ![13017-ATN LP Thumbnail](https://web.genewiz.com/hs-fs/hubfs/TECH%20NOTES%202026/13017-ATN%20LP%20Thumbnail.png?width=884&height=1000&name=13017-ATN%20LP%20Thumbnail.png "13017-ATN LP Thumbnail") ## Download Tech Note ## Related Services [Single-cell Sequencing](https://www.genewiz.com/public/services/next-generation-sequencing/illumina-sequencing) High-resolution transcriptomic profiling from individual cells to reveal cellular heterogeneity [Clinical Services](https://www.genewiz.com/public/services/clinical-services) Regulatory-compliant laboratory testing to support clinical research and trials. [RNA Sequencing](https://www.genewiz.com/public/services/next-generation-sequencing/whole-genome-sequencing) Comprehensive transcriptome profiling to quantify gene expression and transcript diversity.