Optimizing Plasmid Preparation to Increase Yield and Reduce Endotoxins
TECH NOTE
Optimizing Plasmid Preparation to Increase Yield and Reduce Endotoxins
Plasmid DNA (pDNA) is crucial in the fields of gene therapy, vaccine development, and molecular cloning. However, achieving high yields of high-quality pDNA while minimizing endotoxin contamination can be challenging. This tech note will help you significantly increase pDNA yield and dramatically reduce endotoxin levels through advanced protocol improvements, enhancing the efficiency and success of your experiments.
What You'll Learn
- Discover how optimized growth conditions and buffer compositions can boost pDNA yields.
- Learn how refined media and advanced purification techniques can ensure high-purity pDNA with reduced endotoxin levels.
