UNCOVERING CELL TYPE-SPECIFIC EXPRESSION PROFILES IN THE TUMOR MICROENVIRONMENT WITH ULTRA-LOW INPUT RNA-SEQ
Biomedical specimens are often restricted to minute quantities, posing major limitations to RNA-Seq. This case study shows how approximately 50 sorted cells from a glioblastoma can produce transcriptomic data comparable to RNA-Seq experiments that use millions of cells.
- Challenges and solutions for sequencing low-input RNA samples
- Generating high quality sequencing data from <1 ng of RNA
- Analyzing different cell types from the tumor microenvironment